3D Cell Cultures with BIOFLOAT™ Cell Culture Plates

Nice and round! - Spheroid cultures with BIOFLOAT™

The anti-adhesive surface for spheroid culture

Innovative solution

The innovative high-grade anti-adhesive surface of the BIOFLOAT™ cell culture plates acts as an anti-stick coating. The cells form cell-to-cell contacts and aggregate primarily to one very evenly round spheroid per well (>95%). This leads to a high reproducibility of your results.

 How to solve your challenges in the field of spheroid culture:

  • Highly anti-adhesive surface: This enables easy cultivation of uniformly round spheroids with high reproducibility.

  • Fast and reliable results: Uniform spheroids form faster than on most anti-adhesive surfaces, leading to consistent and reliable results.

  • Robust coating: The coating is resistant to washing and scratching, which enables long-lasting and stable use.

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What our BIOFLOAT™ users say


In our BIOFLOAT™ Insights video series, scientists share their personal success stories in 3D cell culture.

Learn more here from our BIOFLOAT™ users.

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BIOFLOAT™ in practice

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Stephen M. McKeon takes endometriosis models to a new level

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M. Benkheil reproduces the complexity of diseases in vitro

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Prof. V. Lauschke reduces the translational gap to clinical application

New study confirms the efficiency of BIOFLOAT™ cell culture plates

In a newly published study, BIOFLOAT™ cell culture plates showed excellent performance in the key quality attribute of single spheroid formation per well with primary human hepatocytes (PHH) in ≥ 95% of wells. It was also shown that the hepatocytes retained key features in culture on BIOFLOAT™, making the model suitable for pharmacological drug discovery. More details and a video interview with Prof Dr Volker Lauschke from Karolinska Institutet can be found here: 

Study and interview

Knowledge centre - Learn more about 3D cell culture and BIOFLOAT™


Convincing performance for convincing results

Test BIOFLOAT™ cell culture plates in your process environment and see the benefits of this pioneering development for yourself:

  • Rounder: novel, highly anti-adhesive surface coating for easy cultivation

  • Faster: uniform spheroids demonstrably form faster than on most anti-adhesive, cell-repellent surfaces

  • More reliable: uniformly round spheroids – typically one per well (>95%) – ensure high reproducibility of your results

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Impressive results: BIOFLOAT™ compared with competitors

Fig.: Per well, 100 µl of a suspension of primary human hepatocytes with a concentration of 25,000 cells/ml were seeded (corresponding to 2,500 cells/well). After spheroid formation, 50 µl of medium were exchanged every 48–72 h.

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Fig.: 200 μl of a suspension of 3T3 cells with a concentration of 30,000 cells/ml (corresponding to 6,000 cells per well) was seeded per well. Wells with exactly one spheroid were identified and expressed as a percentage depending on incubation time.

Fig.: 200 μl of a suspension of 3T3 cells with a concentration of 30,000 cells/ml (corresponding to 6,000 cells per well) was seeded per well. The relative circularity of the spheroids formed was determined and plotted as a function of time. The higher the value, the rounder the spheroid. A value of 1 would correspond to a perfect circle.

Robust – wash- and scratch-resistant coating

Unlike hydrogel-based products, the ultrathin BIOFLOAT™ polymer coating is not affected by washing or mechanical impact from pipette tips. This is also impressively demonstrated by the following scratch test:

Fig.: The bottom of the well was lightly scratched using a standard pipette tip (once around with moderate pressure) and heavily scratched (30 s with strong pressure). Subsequently, 200 µl of a suspension of 3T3 cells with a concentration of 30,000 cells/ml were seeded per well (corresponding to 6,000 cells per well).


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More efficient, faster, more reliable – this is what our users say:

Product Highlights

FAQ

READY. SET. GROW! Cell culture with SARSTEDT

With our extensive product line, we enable excellent cell cultivation in your laboratory. Practical and detailed design: Discover the numerous features of SARSTEDT's bottles, dishes and plates on our landing page. Tailor-made for the needs of most cell types, while meeting the highest quality standards.

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Life is not always science.
But science is our Life.

At SARSTEDT, our work follows the principle: Impress with quality, retain with service. Reliability and experience, innovation and dynamism – we build on proven and reliable values, combined with active processes and partnership-based thinking and action.

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