Product

96 PCR Plate half skirt

Order number: 72.1979.202
96 PCR Plate half skirt

Product description

Order number 72.1979.202
Product description 0.3 ml 96-well Multiply® PCR plate with half lateral skirt for ABI thermocycler and DNA sequencer, transparent, PCR Performance Tested certified, 25 pieces packed in the bag

Product characteristics

Suitable for qPCR Yes
Construction of the plate edges Half
High or low profile High-Profile
Number of wells 96

Size

Sample volume 0,3 ml
Width of product 86 mm
Length of product 126,1 mm

Material & colours

Product material Polypropylen (PP)
Colour of product Transparent

Purity & certification

Product in accordance with MDD or IVDD No marking necessary
CE certified No CE marking
Lot or serial number batched

Packaging

Minimum order qty. 100
Pcs. / inner box 25
Pcs. / box 100
Pcs. / pallet 6400
Case volume 0,0188 cbm
Weight of case 3 KG
EAN of inner box 4038917134958
EAN of outer box 4038917067591
Product specification

General

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    With which cyclers are our PCR plates compatible?

    The following table lists the compatibilities of all common cyclers with our range of PCR plates.

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    What is the difference between plates with 'high-profile' and 'low-profile' well design?

    Low-profile PCR plates and PCR tubes have a low height, minimising the dead volume in the individual wells compared to high-profile products. Therefore the volume of reagents used can be reduced significantly, effectively reducing costs.

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    When should a white PCR plate be used?

    If there is only very minimal starting material and therefore a correspondingly low concentration of cDNA is available. 

    The optimised reflection properties of the wells of white Multiply® PCR plates effectively prevent 'crosstalk' between the wells and increase the fluorescence level of the qPCR reaction by a factor of 10. In this way, reliable Ct values can still be generated through the common qPCR cycles even when there is only a small volume of starting material.

    If you are aiming to achieve the lowest possible variability. 

    The optimised reflection properties of the white Multiply® PCR plates ensure an even and stable fluorescence level of the qPCR reaction. This leads to a significantly improved reproducibility of the measurement results and increased accuracy. Tip: In order to further improve the variability, we recommend processing at least the template DNA and better still all of the reagents used, with our low retention pipette tips. 

    If you are aiming to save reagents. 

    The optimised reflection properties of the wells of white Multiply® PCR plates increase the fluorescence level of the qPCR reaction by a factor of 10. This means that the volume of reagents used can be reduced significantly, effectively reducing costs. 

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    Why should 'PCR Performance Tested' certified Multiply® PCR tubes and plates not be autoclaved?

    In principle, it is possible to autoclave our PCR tubes and PCR plates without affecting the material properties as they are made of polypropylene. However, all Multiply® PCR tubes and PCR plates produced by Sarstedt are manufactured under cleanroom conditions and are free from DNases/RNases, DNA and PCR inhibitors ('PCR Performance Tested'). This means that the most important parameters for a reliable PCR result are present in advance and our PCR tubes and PCR plates can be used for PCR reactions directly. We therefore recommend that you do not autoclave 'PCR Performance Tested' certified PCR tubes and plates because, contrary to the intention of this step, it results in a risk of contaminating the materials with nucleic acids. 

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    Can our Multiply® PCR tubes and Multiply® PCR plates be frozen or stored in cold storage rooms?

     

    In principle, Multiply® PCR tubes and Multiply® PCR plates can be frozen as they are made of polypropylene. However, the freezing conditions must be selected such that the content can be evenly frozen or frozen from the bottom to the top. Cracks caused by strain can occur in the lower part if the upper part of the tube freezes first as a result of unfavourable freezing conditions. For example, depending on the diameter of the hole, stands made out of Styrofoam have a strongly insulating effect which makes it more difficult to ensure the even freezing of the samples. Due to the large number of possible influence factors (the load on the tubes can be very different depending on the medium to be frozen and the routine conditions), we recommend you carry out a freezing test under your routine conditions. 

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    Which lids and foils can be used for qPCR?

    We offer special transparent lid strips (65.1998.400) and a selection of transparent foils (95.1994, 95.1999) for qPCR. Items with frosted lids in the PCR strips offer the advantage of improved labelling and are suitable for standard PCR applications.  

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