BIOFLOAT™ spheroid culture

BIOFLOAT™ spheroid culture

The new BIOFLOAT™ cell culture surface for spheroid culture features a high-grade anti-adhesive property. This makes it possible for the cultivated cells to form primarily cell-cell contacts without adhering to the surface of the vessel. BIOFLOAT™ thus makes it possible for you to produce perfect spheroids quickly and reproducibly. 

BIOFLOAT™ is used in a variety of fields of biomedical research such as cancer and stem cell research, in the preclinical phase of drug research and in toxicological studies. The efficiency and reliability of preclinical cell models are improved for these applications.

General

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    Where do you find the plate dimensions that are needed for use in an analysis device?

    The technical drawing can be found under the “Technical Information” tab.

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    What cell count should be seeded per well?

    The initial cell count selected depends on the cell type/cell line and the desired size of the spheroids. Experience has shown that cell counts between 1,000 and 6,000 cells/well are suitable. To set the cell count per well for the respective experiment, it is recommended to carry out a dilution series and then determine the optimal cell concentration based on the desired parameters.

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    Do the plates need to be centrifuged after seeding the cells?

    A centrifugation step is not recommended. Unnecessary centrifugation can even have a negative effect on spheroid formation. However, if you wish to centrifuge the cells at the start anyway, 200-300 x g for 2-5 min. should not be exceeded. 

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    How quickly do spheroids form in the plates?

    The time required by the cells to form spheroids depends on the cell line. With BIOFLOAT™, formation has been observed after 2-24 h. It should be noted that some cells first form only loose aggregates that become increasingly dense over time, so the diameter of the spheroid may first become smaller in this initial phase. 

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    Why do some cells form only loose spheroids?

    Not all cell lines or cell types are equally suitable for forming spheroids. It may be helpful to add proteins of the extracellular matrix to make spheroid formation possible. 

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    How long can cells be cultivated in the plate?

    The maximum possible period for spheroid culture depends on the cell line. Cells have been cultivated continuously for more than 80 days in the BIOFLOAT™ 96-well plate. 

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    Are the BIOFLOAT™ plates suitable for cultivating organoids?

    Spheroids, which usually consist of one or maximum two cell types/lines, should not be confused with organoids, which are more complex 3D cell cultures. The formation of organoids is a complex process that cannot be reproduced in the BIOFLOAT™ plate alone. However, previously formed organoids have been successfully cultivated in the BIOFLOAT™ plate. 

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    Can the medium in the plate be changed without using a new plate?

    The BIOFLOAT™ surface is very robust against mechanical effects caused by pipetting. It is thus no problem to change the medium in the plate several times. 

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