FAQs - Frequently asked questions

FAQ

Below, to assist you we have collated the most usefull frequently asked questions (FAQs). You didn't find an answer to your question? Please contact us.

Life Science // Sample handling

Screw cap micro tubes

Can screw cap micro tubes be stored on dry ice?

Is there a recommended torque for micro tube screw caps?

Can screw cap micro tubes be used at temperatures below zero centigrade?

What are the maximum speeds at which screw cap micro tubes can be centrifuged?

Can screw cap micro tubes and reaction tubes be autoclaved?

What are the dimensions of screw cap micro tubes?

Reaction tubes

Can screw cap micro tubes and reaction tubes be autoclaved?

What are the maximum speeds at which the reaction tubes can be centrifuged?

Reagent and centrifuge tubes

Can polypropylene tubes be used for freezing?

Can reagent and centrifuge tubes be autoclaved?

What are the maximum speeds at which the reagent and centrifuge tubes can be centrifuged?

How exact is the scale on the 15 ml and 50 ml tubes?

Caps

Can products made of polyethylene be autoclaved?

Life Science // Liquid handling

Pipette tips

When should a filter tip be used?

Can very small volumes be processed precisely with SARSTEDT pipette tips?

Can the pipette tips with filters be autoclaved?

When should a low retention pipette tip be used?

Which pipette tips are compatible with which pipettes?

Which pipette models are compatible with the refill tips?

How do I feed the boxes and refill components into the SARSTEDT recycling process?

Serological pipettes

Why do the serological pipettes have a negative graduation which goes beyond the volume indicated?

What is the dose accuracy of the Sarstedt serological pipettes?

Life Science // Cell culture

Cell culture flasks, plates and dishes

What does the red-yellow-green colour code with which the cell culture products are labelled mean?

Which caps are available with the cell culture flasks?

When should each type of cap be used for cell culture flasks?

Can the SARSTEDT cell culture flasks, plates and dishes be coated with, for example, poly-L-lysine?

What sizes of cell culture vessels does SARSTEDT offer?

What is the difference between primary and secondary cells?

What is the difference between suspension cells and adherent cells?

Which cell types are best suited for the respective growth surfaces?

How long do the growth surfaces remain stable under different cultivation conditions?

Are there special handling instructions for the culture of particularly sensitive cells?

Why do my cells grow in patterns (ring or wave-like) ?

BIOFLOAT™ cell culture plate, spheroid culture

What is the difference between primary and secondary cells?

What is the difference between suspension cells and adherent cells?

Can the medium in the plate be changed without using a new plate?

Are the BIOFLOAT™ plates suitable for cultivating organoids?

How long can cells be cultivated in the plate?

Why do some cells form only loose spheroids?

How quickly do spheroids form in the plates?

Do the plates need to be centrifuged after seeding the cells?

What cell count should be seeded per well?

Where do you find the plate dimensions that are needed for use in an analysis device?

Is there a protocol for spheroid culture in the BIOFLOAT™ plate?

Can fluorescence images be taken in the BIOFLOAT™ plate?

Which cell types are best suited for the respective growth surfaces?

How long do the growth surfaces remain stable under different cultivation conditions?

lumox® optical foil base

Which products are suitable for carrying out fluorescence or luminescence-based measurements of cells?

lumox® products are suitable for the cultivation of which cells?

What is lumox®?

Are the cell culture products checked for the presence of pyrogens or cytotoxins?

x-well microscope slide

What is the maximum magnification at which the different x-well tissue culture chambers can optimally be viewed under the microscope?

In which x-well tissue culture chambers is the slide detachable?

What are x-well products and what can they be used for?

miniPERM® bioreactor

How is the miniPERM® bioreactor subdivided?

In which sizes and designs are the miniPERM® bioreactors available?

What are the advantages of using a miniPERM® bioreactor?

Cryopreservation

How are the SARSTEDT CryoPure tubes certified?

Where should the CryoPure tubes in the nitrogen tank be stored – in the liquid phase or in the gas phase?

Filtration

With which connections are Filtropur L filters available?

What are the characteristics of Filtropur S, Filtropur S plus and Filtropur L filters?

Are the Filtropur BT filter attachments compatible with the threads of other bottles?

Which filtration units can be used to remove mycoplasms from solutions?

What pore size filter materials are available at SARSTEDT?

Life Science // Microbiology

Petri dishes

What can Petri dishes with two compartments be used for?

What are the advantages of Petri dishes with and without ventilation cams?

Are Sarstedt Petri dishes suitable for use in automated plate casting machines?

Swabs

What are the advantages of swabs with charcoal in the transport medium?

What are swabs with transport medium suitable for?

What can swabs without medium be used for?

Can the 80.625 swab be autoclaved?

What can the swabs with transport medium be used for?

Petri dish racks

What plastics are the SARSTEDT DishRack Petri dish stands made from and can these racks generally be autoclaved?

Inoculation tools

What are the advantages of SARSTEDT inoculation loops, inoculation needles and inoculation spreaders and for which applications are they suitable?

How high is the accuracy of the 1 µl and 10 µl inoculation loops?

Life Science // PCR and molecular biology

PCR plates, strips and single tubes

Which lids and foils can be used for qPCR?

Can Sarstedt's Multiply® PCR tubes and Multiply® PCR plates be frozen or stored in cold storage rooms?

Why should 'PCR Performance Tested' certified Multiply® PCR tubes and plates not be autoclaved?

When should a white PCR plate be used?

What is the difference between plates with 'high-profile' and 'low-profile' well design?